Molecular weight: recombinant human TGF-β3 is a 27.2 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulfide bond.
Description:Transforming growth factor –β is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homodimeric proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGFβ (TGFβ-1, TGFβ-2 and TGFβ-3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing.
Figure 1. SDS-PAGE analysis of recombinant TGF beta3. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane MWM: Molecular weight marker (kDa); Lane 1: commercial recombinant TGF beta3 with no tag. Lane 2: contains 300 ng of 6-His-tag Agrenvec´s recombinant TGF beta3.
Figure 2. Western Blot analysis of recombinant TGF-β3. Lane MWM: Molecular weight marker (kDa); Lane 1: commercial recombinant TGF β3 with no tag. Lane 2: contains 300 ng of 6-His-tag Agrenvec´s recombinant TGF β3.
Human recombinant protein expressed in Nicotiana benthamiana.
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