Molecular weight: recombinant human TGF-β2 is a 27.08 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulfide bond.
Figure 1. SDS-PAGE analysis of recombinant TGF-β2. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. MWM: Molecular weight marker (kDa); Lane 1 contains a commercial recombinant TGF-B2 (no His-Tag. Lane 2-3 contain 250-300 ng of 6-His-Tag Agrenvec´s recombinant TGF-β2.
Figure 2. Western Blot analysis of recombinant TGF-beta2. MWM: Molecular weight marker (kDa); Lane 1: commercial recombinant TGF-beta2 (no His-tag); Lane 2-3: contains 250-500 ng of 6-His-tag Agrenvec´s recombinant TGF-beta2.
Biological Activity: The biological activity of TGF-β2 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. ED50 ≤ 50ng/mlHuman recombinant protein expressed in Nicotiana benthamiana.
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