Molecular weight: rhuman sRANKL is a glycosilated polypeptide chain containing 175 amino acids (70 – 244 aa of O14788 TNF11_HUMAN) and a His-tag at the N-terminal end. It has a predicted molecular mass of 21.1 kDa, however as result of glycosilation, the recombinant protein could migrate as two bands with an apparent molecular mass of 21-23 kDa in SDSPAGE.
Description: Recombinant human RANKL is a member of TNF super family, a cytokine that play a central role in bone remodeling and disorders of mineral metabolism. It was shown to be a dendritic cell survival factor, T-cell activator and osteoclast regulator because RANKL mediates the osteoclast differentiation, survival and activation. Native RANKL is a type II trans-membrane protein with an extracellular binding domain that interacts with RANK and OPG receptors. OPG protects the skeleton from excessive bone resorption by binding to RANKL and preventing it from binding to its receptor, RANK. Thus, RANKL/OPG ratio became an important determinant of bone mass and skeletal integrity. In addition, this protein was shown to activate anti-apoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TREAF). Recent findings shown that OPG/RANK/RANKL system has been identifies as a possible mediator of arterial calcification suggesting common links between osteoporosis and vascular diseases.
Figure 1.- SDS-PAGE and Western blot analysis of human recombinant sRANKL. Lane MWM: molecular weight marker (kDa). Lane1: coomassie blue 1 µg of recombinant sRANKL and lane 2: 0,1 µg of recombinant sRANKL. All the bands shown in lanes 1-2 have been identify by MALDI-TOFF as recombinant sRANKL.
Biological Activity: The specific activity is determined by the dose-dependent stimulation of IL-8 production in human PBMC. Activity results may vary with PBMC donors. Human PBMC stimulated with 10ng/ml of Human recombinant sRANKL increases in three times the basal production of IL-8.
Human recombinant protein expressed in Nicotiana benthamiana.
Bulk sizes are available upon request.
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