Molecular weight: rhuman sRANKL is a glycosylated polypeptide chain containing 175 amino acids (70 – 244 aa of O14788 TNF11_HUMAN) and a His-tag at the N-terminal end. It has a predicted molecular mass of 21.1 kDa, however as result of glycosylation, the recombinant protein could migrate as two bands with an apparent molecular mass of 21-23 kDa in SDSPAGE.
Description: Recombinant human RANKL is a member of TNF super family, a cytokine that play a central role in bone remodelling and disorders of mineral metabolism. It was shown to be a dendritic cell survival factor, T-cell activator and osteoclast regulator because RANKL mediates the osteoclast differentiation, survival and activation. Native RANKL is a type II trans-membrane protein with an extracellular binding domain that interacts with RANK and OPG receptors. OPG protects the skeleton from excessive bone resorption by binding to RANKL and preventing it from binding to its receptor, RANK. Thus, RANKL/OPG ratio became an important determinant of bone mass and skeletal integrity. In addition, this protein was shown to activate anti-apoptotic kinase AKT/PKB through a signalling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TREAF). Recent findings shown that OPG/RANK/RANKL system has been identifies as a possible mediator of arterial calcification suggesting common links between osteoporosis and vascular diseases.
Figure 1.- SDS-PAGE analysis of recombinant sRANKL. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane MWM: Molecular weight marker (kDa); lane 1 contains 1 ug of rhuman sRANKL. All bands have been identified by MALDI-TOFF as human RANKL.
Figure 2.- Analysis of rhuman sRANKL with specific antibody by Western Blot; Lane MWM: Molecular weight marker (kDa); lane 1 contains 1 ug; lane 2 contains 0.5 ug and lane 3 contains 0.1 ug of rhuman sRANKL . All bands have been identified by MALDI-TOFF as human RANKL.
Biological Activity: Not available.Human recombinant protein expressed in Nicotiana benthamiana.
Bulk sizes are available upon request.
For R&D purposes only. Purchaser must determine the suitability of the product(s) for their particular use.