Molecular weight: rHuman Interleukin 7 is a glycosilated polypeptide chain containing 160 amino acids (26 -177 of P13232 IL7_HUMAN ), fused to His tag at N-terminal. rHuman IL-7 migrate at approximatly 18 – 25 kDa by SDS-PAGE.
Description: Interleukin-7 (IL-7) is a member of the interleukin family which regulates the development and survival of lymphoid and myeloid cells. It has a potent effect for the stimulation of the adaptative immune response, it promotes proliferation of lymphoid progenitors (Pre-B, Pro-B and early T cells), and the effects include mobilization, maturation, and maintenance of IL-7 responsive immune cells. The biological functions of IL-7 as a key ‘‘lymphotrophin” in JAK-STAT pathways, PI3 kinase pathway, and Src kinase pathway. IL-7 has also been shown to play a vital role in energy metabolism of T cells and regulating glucose uptake in T lymphocytes through Glut1 via activation of STAT5 and Akt. It has a thymic antiapoptotic effect, and induce the expression of antiapoptotic proteins Bcl-2 and Mcl-1 and the inhibition of preapoptotic proteins Bax and Bad. IL-7 has potential therapeutic applications for the lymphoid reconstitution of immuno-depressed patients (chemotherapy, radiation, AIDS, and bone marrow transplant).
Figure 1. SDS-PAGE and Western Blot analysis of recombinant Il-7. Samples were loaded in 15% SDS-polyacrylamide gel and stained with coomassie blue. Lane 1: Molecular weight marker (kDa); Lane 2 -4: contains 0.5, 0.25 and 0.1 ng of Agrenvec recombinant IL-7. Lane 5 -7: immune-detection with specific anti-human IL-7 of 0.5, 0.25 and 0.1 ng respectively. All bands shown in lane 2-4 have been identifying by MALDI-TOFF as recombinant IL-7.
Biological Activity: The activity of recombinant Human Interleukin-7 is measured in a cell proliferation assay using PHA activated human peripheral blood lymphocytes. *Cell proliferation was measured by MTT method. The ED50 for this effect is typically <10ng/ml in presence of goat anti-human IgM (µ chain specific).
Human recombinant protein expressed in Nicotiana benthamiana.
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