Active rh Interleukin-6 – RF0032

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RF0032
  • Description: Active human Interleukin-6
  • Application: BIOASSAY, SDS-PAGE, WB
  • Alternative Names: IL-6, B-cell stimulatory factor 2, Hybridoma growth factor, Interferon beta-2
  • UniProtKB: P05231
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Description

Molecular weight: rhuman Interleukin-6 is a  polypeptide chain containing 183  amino acids (30 – 212 of  P05231 IL6_HUMAN), and 10 aa Histidine-based tag. It as a predicted molecular mass of 22.2 kDa,  however as result of potential glycosilation, the recombinant protein could migrate with an apparent molecular mass of 23-24 kDa in SDSPAGE.

Description: rhuman IL-6 is an important pro-inflammatory and anti-inflammatory cytokine expressed by  many types cell including: T and  B cells, macrophages, endothelial cells, fibroblasts,  monocytes , keratinocy and certain tumor cells. It Is a multifunctional cytokine that   modulate several physiologic processes such as hematopoiesis, stimulation of immunoglobulin synthesis, maturation and activated B cells, differentiation of T lymphocytes   and regulation of the hepatic acute-phase response. IL-6 is also produced by muscle, is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. The binding of il-6 to its receptor triggers the association of a hetheromeric IL-6R/gp130 complex,   which activates JAK/ STAT1 cascade. In a mechanism known as trans- signaling, complex of soluble   IL-6/IL-6R elicit responses from gp130-expressing cells that lack cell surface IL-6R, this enable a wider range of cell types to respond to IL-6.

Western-Blot-analysis-of-recombinant-IL-6-RF0032-131x300
Figure 1. Western Blot analysis of recombinant IL-6. MWM:  Molecular weight marker (kDa); Lane 1 contains 300 ng of  recombinant IL-6.

Biological Activity: The specific activity is determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erytroleukemic indicator cell line). ED50 < 1 ng/ml

RF0032-The-specific-activity-IL-6-300x180

Human recombinant protein expressed in Nicotiana benthamiana.

Bulk sizes are available upon request.

For R&D purposes only. Purchaser must determine the suitability of the product(s) for their particular use.