Molecular weight: rhuman Interleukin-3 is a glycosylated polypeptide chain containing 133 amino acids (20 – 152 of P08700 IL3_HUMAN) and a His-tag at the N-terminal end. It has a predicted molecular mass of 16.2 KDa, however as result of glycosylation, the recombinant protein could migrate with an apparent molecular mass of 18-22 kDa in SDSPAGE.
Description: IL-3 is a potent growth factor involved in a variety of cell activities such as cell growth, differentiation and apoptosis.
It takes part of several biological responses such as proliferation, and differentiation of a broad range of hematopoietic progenitor cells into erythrocytes, granulocytes, monocytes, megakaryocytic and mast cells. IL-3 also induces the production of several enzymes involved in cellular metabolism, differentiation, and DNA/RNA metabolism.
IL-3 is produced by activated T-lymphocytes, keratinocytes, NK-cells, mast cells, endothelial cells and monocytes. The biological activity of IL-3 is mediated through specific cell surface receptor that is composed of alpha and beta subunits. Alpha subunit is responsible for the binding and beta subunit transmits signals across the plasma membrane; il-3 is known to activate three signalling pathways: JAK/STAT, RAS/RAF/MAP kinase, and the PI-3kinase/PKB pathways.
IL-3 is also implicated in the pathogenesis of several diseases such as asthma, athero sclerosis and multiple sclerosis. Recombinant protein has been widely used in clinical practice, in the treatment of leukemia and as therapy for patients with bone marrow deficiency function.
Figure 1. SDS-PAGE analysis of recombinant IL-3. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane MWM: Molecular weight marker (kDa); Lane-1 contains 0.15 ug and lane-2 contains 0.3 ug of rhuman IL-3.
Figure 2.- Analysis of rhuman IL-3 with specific antibody by Western Blot; Lane MWM: Molecular weight marker (kDa); Lane-1 contains 0.15 ug and lane-2 contains 0.3 ug of rhuman IL-3.
Biological Activity: The specific activity is determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erytroleukemic indicator cell line). ED50 of Agrenvec´s rHuman IL-3 is typically less than 1 ng/ml.
Human recombinant protein expressed in Nicotiana benthamiana.
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