Molecular weight: Recombinant human IFN-alpha-2a is a polypeptide single chain containing 171 amino acids with a predicted molecular mass of 20.1 kDa. Recombinant human IFN-alpha-2 contains a His-tag at the C-terminal end.
Description: Interferon alpha 2a is an interferon class I produced by the cells of the innate immune system as a part of the defense response to foreign agents such as viruses, parasites and tumor cells. The human alpha-interferon family displays broad spectrum antiviral, antiproliferative and immunomodulatory activities on a variety of cell types. Interferon Alpha-2a (IFNa-2a), one of the subtypes, is a protein consisting of 165 amino acid. Its three-dimensional loop structure is maintained by two disulfide bridges. It has an approximate molecular weight of 20 kDa.
Figure 1.SDS-PAGE and Western blot analysis of human recombinant IFN-alpha-2a. Lane MWM: molecular weight marker (kDa). Lane1: coomassie blue 0,3 µg of recombinant IFN-alpha-2a and lane 2: 0,3 µg of recombinant IFN-alpha-2a.
Biological Activity: The specific activity was determined by a quantitative gene reported bioassay using human Type I interferon-sensitive cells. The activity is < 8 X 108 units/mg in reference to a viral resistance assay using bovine kidney MDBK cells.
Human recombinant protein expressed in Nicotiana benthamiana.
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