Molecular weight : Recombinant human Follistatin isoform 2 is a polypeptide chain containing 217 amino acids (30–217 of P19883 FST_HUMAN). It has a predicted molecular mass of 32.3 kDa.
Description: Follistatin is a monomeric glycoprotein that binds to ligands of the TGF-β superfamily and regulates their activity by inhibiting their access to signalling receptors. Follistatin received its name because it suppresses synthesis and secretion of follicle-stimulating hormone (FSH) from the pituitary gland. The follistatin gene localizes to chromosome 5q11.2. It is composed of a relatively small 6-kb genomic DNA consisting of six exons. There is an alternative splice site that generates two major species, a full-length version that encodes a 344-amino acid preprotein differing by a 27-amino acid sequence from its carboxy-shortened version of the 317-amino acid form missing exon 6. Prior to activation, follistatin, like myostatin, undergoes further posttranslational modification to lose another 29 amino acids by removal of the signal peptide that results in polypeptides of 315 (FS315), often referred to as the long isoform and 288 (FS288), called the short isoform.
Figure 1. SDS-PAGE and Western blot analysis of human recombinant Follistatin. Lane MWM: molecular weight marker (kDa). Lane 1-3 coomassie blue 0.3 , 0.2 and 0.1µg of recombinant Follistatin; lane 4-6 : 0.3, 0.2 and 0.1 µg of recombinant Follistatin.
Biological Activity: The biological activity of human Growth Hormone is measured by cell proliferation using Nb2-11 cells. ED50 is 0.1-0.3 ug/ml in the presence of 7.5 ng/ml Activin A.
Human recombinant protein expressed in Nicotiana benthamiana.
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