Molecular weight: recombinant human B lymphocyte activating factor (BAFF) is a glycosilated polypeptide chain containing 151 amino acids (134-285 aa Q9Y275 (TN13B_HUMAN) fused to 10 His tag at N-terminal. rHuman BAFF has a molecular mass betwen 18 and 20kDa.
Description: BAFF (B lymphocyte activating factor) is a member of the tumor necrosis factor (TNF) ligand family which is expressed in T Cells, macrophages, monocytes and dendritic cells. It is also known as BLyS, THANK, TALL, zTNF4 and TNFS20. BAFF enhances B cell survival in vitro and has emerged as a key regulator of peripheric B cell and it is vital homeostatic cytokine for B cells that helps regulate both innate and adaptive immune responses. BAFF binds to three TNF receptors: B-cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium modulator and cyclophilin ligand interactor(TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C).The human BAFF gene code for a 285 amino acids type II transmembrane protein. Recombinant human soluble BAFF is a 151 amino acids containing the TNF-like portion of the extracellular domain of BAFF.
Figure 1. Lanes1-2:SDS-PAGE analysis of recombinant BAFF. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane 1: Molecularweightmarker(kDa); Lane2:contains 300ng of rhuman BAFF. Lane3: Analysis of recombinant BAFF with specific anti-human BAFF by Western Blot; contains 300ng of rhumanBAFF. All bands shown in lane2-3 have been identify by MALDI-TOFF as recombinant BAFF
Biological Activity: The activity is determined by dose-dependent response of proliferation Anti IgG M stimulated B cell from Human PBMC. Cell proliferation was measured by MTT method. *activity results may vary with PBMC donors. The ED50 for this effect is typically <10ng/ml in presence of goat anti-human IgM (µ chain specific).
Human recombinant protein expressed in Nicotiana benthamiana.
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