Molecular weight: 14 KDa βB single chain, containing 123 amino residues.
Description: Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGF-beta family. Mature Activin B has two chains of 123 amino acids residues (betaB-betaB). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Inhibins/Activins are protein that are formed by the dimerization of two subunits, i. e. an α (alpha) with either beta A (βA) – Inhibin A or beta B (βB) – Inhibin B. The subunits betaA and betaB can also form homodimers or heterodimers called Activin: Activin A (betaA -betaA), Activin B (betaB -betaB) and Activin AB (betaA -betaB). The Activin gene family comprises the additional, but poorly characterized members’ Activin betaC (βC), beta D (βD) and beta E (βE). As with other members of the super-family, Activins interact with two types of cell surface trans-membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors, ACVR2A, ACVR2B.
Figure 1. SDS-PAGE analysis of recombinant Activin B. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane 1: Molecular weight marker (kDa); Lane 2: contains 0.5 ug of recombinant Activin B.
Figure 2. Western blot analysis of recombinant Activin B. Lane 1: Molecular weight marker (kDa). Lane 2: 0.2. ug of Activin B.
Biological Activity: The biological activity of Activin B is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. EC50 < 5 ng/mL are required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.Human recombinant protein expressed in Nicotiana benthamiana. Bulk sizes are available upon request. For R&D purposes only. Purchaser must determine the suitability of the product(s) for their particular use.