Description: Serologic cualitative detection of rh TGFbeta-3 using Anti-Human TGFbeta-3 IgG. Developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).
Immunogen: Highly pure (>97%) recombinant human TGFbeta-3 expressed in plants.
- Western Blot: Suggested starting dilution 1/500. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
Fig 1. Western Blot analysis of rhumanTGF beta-3 using Anti-Human TGFbeta-3 IgG (RF0018)
Human TGFbeta-3 protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:1,000 of Anti-Human TGFbeta-3 IgG. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM (kDa); Lane 2: 0.1 ug of rHuman TGFbeta-3 protein.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.