Description: Serologic cualitative detection of rh TFPI-2 using Anti-Human TFPI-2 IgG developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).
Immunogen: Highly pure (>97%) recombinant human Kunitz domain 1 from TFPI-2 expressed in plants.
- Western Blot: Suggested starting dilution 1/500. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
- ELISA: Suggested starting dilution 1/300. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
Fig 1.Western Blot analysis of rhuman TFPI-2 using Anti-Human TFPI-2 IgG (RF0012)
Human TFPI-2 protein was resolved by SDS-PAGE, transferred to a NC membrane. Both membranes were probed with a dilution 1:500 of Anti-Human TFPI-2 IgG. Anti-rabbit IgGAP (alkaline phosphatase conjugate) was used as secondary reagent. A.-Lane 1: MW marker(kDa). Lane 2: 0,1 ug of recombinant Kunitz domain 1 produced in plants. ; B.- Lane 1; MW marker (kDa). Lane 2: 0,25 ug of Human TFPI-2.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.