Description: Serologic cualitative detection of rh LXR beta using AntiHuman LXR beta IgG. Developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).
Immunogen: Highly pure (>97%) recombinant human LXR beta LBD (ligand binding domain)expressed in plants.
- Western Blot: Suggested starting dilution 1/1,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
Fig 1. Western Blot analysis of rhuman LXR using Anti-Human LXR beta IgG (RF0020)
Human LXR beta protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:1,000 of Anti-Human LXR beta IgG. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 0.2 ug of rHuman LXR protein.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.