Description: Serologic cualitative detection of rh IL-6 using Anti-Human IL-6 IgG is developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).
Immunogen: Highly pure (>97%) recombinant human IL-6 expressed in plants.
- Western Blot: Suggested starting dilution 1/1,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
- ELISA: Suggested starting dilution 1/500. This antibody, in conjunction with compatible secondary reagents (anti-rabbit IgG-AP conjugate), allows the detection of <10 ng/well of rhuman IL-6 (RF0032).
Fig 1. Western Blot analysis of rhuman IL-6 using Anti-Human IL-6 IgG (RF0056)
Human IL-6 protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:1,000 of Anti-Human IL-6 IgG. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 0.25 ug f non glycosylated rHuman IL-6 protein produced in a prokaryotic expression system. Lane 3: 0.25 ug of glycosylated isoforms of rH IL-6 produced in an eukaryotic expression system.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.