Easy blot Mini YKit HGH – RF0071

160€

SKU:
RF0071
  • Antibody Source: Rabbit
  • Clonality: Polyclonal-IgG
  • Packaging:
  • Anti-Human Growth Hormone (IgG) 100 ug lyophilized (RF0014)
  • Recombinant Human Growth Hormone Active 1 ug lyophilized (RF0011)
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Description

Description: Serologic cualitative detection of rh GH using Anti-Human Growth Hormone IgG is developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).

Immunogen: Highly pure (>97%) recombinant human Growth Hormone expressed in plants.

Applications:

  • Western Blot: Suggested starting dilution 1/1,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
  • ELISA: Suggested starting dilution 1/500. This antibody, in conjunction with compatible secondary reagents (anti-rabbit IgG-AP conjugate), allows the detection of < 10 ng/well of rhuman Growth Hormone protein (Agrenvec RF0011).

 

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Fig 1. Western Blot analysis of rhuman Growth Hormone using Anti-Human Growth Hormone IgG (RF0014)
Human Growth Hormone protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:1,500 of Anti Human Growth Hormone IgG. Antirabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 0.3 ug of rHuman Growth Hormone protein.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.