Description: Serologic cualitative detection of rh GH using Anti-Human Growth Hormone IgG is developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).
Immunogen: Highly pure (>97%) recombinant human Growth Hormone expressed in plants.
- Western Blot: Suggested starting dilution 1/1,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
- ELISA: Suggested starting dilution 1/500. This antibody, in conjunction with compatible secondary reagents (anti-rabbit IgG-AP conjugate), allows the detection of < 10 ng/well of rhuman Growth Hormone protein (Agrenvec RF0011).
Fig 1. Western Blot analysis of rhuman Growth Hormone using Anti-Human Growth Hormone IgG (RF0014)
Human Growth Hormone protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:1,500 of Anti Human Growth Hormone IgG. Antirabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 0.3 ug of rHuman Growth Hormone protein.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.