Easy blot Mini YKit Activin-B – RF0075

160€

SKU:
RF0075
  • Antibody Source: Rabbit
  • Clonality: Polyclonal-IgG
  • Packaging: 
  • Anti-Human Activin B (IgG) 100 ug lyophilized (RF0054)
  • Recombinant Human Activin B 1 ug lyophilized (RF0029)
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Description

Description: Serologic cualitative detection of Activin B using Anti-Human Activin B IgG, developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).

Immunogen: Highly pure (>97%) recombinant human Activin B expressed in plants.

Applications:

  • Western Blot: Suggested starting dilution 1/500. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
  • ELISA: Suggested starting dilution 1/500. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of < 10 ng/well of rhuman Activin B (RF0029).

RF0054-WB-Anti-Human Activin B (IgG)

Fig 1. Western Blot analysis of rhuman Activin B using Anti-Human Activin B IgG (RF0054)
Human Activin B protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:500 of Anti-Human Activin B IgG. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. MWM: molecular weight marker; Lane 1: 0.1 ug of rH Activin B (monomeric form); Lane 2: 0.3 ug of rH Activin B (multimeric form).
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.