Easy blot Mini YKit Activin-A – RF0074

160€

SKU:
RF0074
  • Antibody Source: Rabbit
  • Clonality: Polyclonal-IgG
  • Packaging: 
  • Anti-Human Activin A (IgG) 100 ug lyophilized
  • Recombinant Human Activin A 1 ug lyophilized
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Description

Description: Serologic cualitative detection of rh Activin A using AntiHuman Activin A IgG developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).

Immunogen: Highly pure (>97%) recombinant human Activin A expressed in plants.

Applications:

  • Western Blot: Suggested starting dilution 1/500. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
  • ELISA: Suggested starting dilution 1/500. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of 0.2-1 ng/well of rhuman Activin A Active (RF0010).
  • Neutralization: To yield one-half maximal inhibition (ND50) of

1

Fig 1. Western Blot analysis of rhuman Activin A using Anti-Human Activin A IgG (RF0016)
Human Activin A protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:1,000 Anti-Human Activin A IgG. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. MWM; Lane 1: 0.2 ug of rH Activin A protein.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.