Description: Serologic cualitative detection of rh IL-6 using Anti-Human IL-6 Serum is developed in rabbit using recombinant IL-6 produced in plants.
Immunogen: Highly pure (>97%) recombinant human IL-6 expressed in plants.
- Western Blot: Suggested starting dilution 1/2,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
- ELISA: Suggested starting dilution 1/2,000. This antibody, in conjunction with compatible secondary reagents (anti-rabbit IgG-AP conjugate), allows the detection of < of 10 ng/well of rhuman IL-6 (RF0032).
Fig 1. Western Blot analysis of rhuman IL-6 using Anti-Human IL-6 Serum (RF0044)
Human IL-6 protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:4,000 of Anti-Human IL-6 Serum (RF0044). Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 1 ug of glycosylated isoforms of rH IL-6 produced in an eukaryotic expression system. Lane 3: 0.25 ug of non glycosylated rHuman IL-6 protein produced in a prokaryotic expression system.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.