Easy blot Mini SKit IL-12 – RF0089

210€

SKU:
RF0089
  • Antibody Source: Rabbit
  • Clonality: Polyclonal
  • Packaging: 
  • Anti-Human IL12p40 (Serum) 100 μl lyophilized (RF0045)
  • Recombinant Human IL-12p40 1 ug lyophilized (RF0031)
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Description

Description: Serologic cualitative detection of rh IL12p40 using Anti-Human IL12p40 Serum is developed in rabbit using recombinant IL12p40 produced in plants.

Immunogen: Highly pure (>97%) recombinant human IL12p40 expressed in plants.

Applications:

  • Western Blot: Suggested starting dilution 1/10,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
  • ELISA: Suggested starting dilution 1/1,000. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of < 10 ng/well of rhuman IL12p40 (RF0031).

3

 Fig 1. Western Blot analysis of rhuman IL12p40 using Anti-Human IL12p40 Serum (RF0045)

Human IL12p40 protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:10,000 of Anti-Human IL12p40 Serum. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1 : MWM; Lane 2: 0.25 ug of glycosylated isoforms of rHuman IL-12p40 produced in an eukaryotic expression system. Lane 3: 0.25 ug of non glycosylated rHuman IL-12p40 protein produced in a prokaryotic expression system.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.