Easy blot Mini SKit HGH – RF0084

210€

SKU:
RF0084
  • Antibody Source: Rabbit
  • Clonality: Polyclonal
  • Packaging: 
  • Anti-Human Growth Hormone (Serum) 100 μl lyophilized (RF0049)
  • Recombinant Human Growth Hormone Active 1 ug lyophilized (RF0011)
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Description

Description: Serologic cualitative detection of HGH using Anti-Human Growth Hormone Serum is developed in rabbit using recombinant Growth Hormone produced in plants.

Immunogen: Highly pure (>97%) recombinant human Growth Hormone expressed in plants.

Applications:

  • Western Blot: Suggested starting dilution 1/5,000. Anti-rabbit IgG-AP(alkaline phosphatase conjugate) is recommended as secondary reagent.
  • ELISA: Suggested starting dilution 1/4,000. This antibody, in conjunction with compatible secondary reagents (anti-rabbit IgG-AP conjugate), allows the detection of < 5 ng/well of rhuman Growth Hormone protein (Agrenvec RF0011).

1

Fig 1. Western Blot analysis of rhuman Growth Hormone using Anti-Human Growth Hormone Serum (RF0049)
Human Growth Hormone protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:10,000 of Anti-Human Growth Hormone Serum. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM. Lane 2: contains 0,05 ug of rHuman Growth Hormone protein.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.