Description: Anti-Human sRANKL Serum is developed in rabbit using recombinant sRANKL produced in plants.
Immunogen: Highly pure (>97%) recombinant human sRANKL expressed in plants.
- ELISA: Suggested starting dilution 1/4,000. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of > 0,5 ng/well of rhuman sRANKL (RF0038)
- Western Blot: Suggested starting dilution 1/4,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
Fig 1. Western Blot analysis of rhuman sRANKL using Anti-Human sRANKL Serum (RF0096)
Human sRANKL protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:4,000 of Anti-Human sRANKL serum. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM (kDa); Lane 2: 0.5 ug of glycosylated isoforms of rHuman sRANKL protein produced in an eukaryotic expression system. Lane 3: 0.1 ug of non glycosylated rHuman sRANKL protein produced in a prokaryotic expression system.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.Optimal dilution conditions should be determined by the final user. Bulk sizes are available upon request. For R&D purposes only. Purchaser must determine the suitability of the product(s) for their particular use.