Anti-Human Growth Hormone (Serum) – RF0049

150€

SKU:
RF0049
  • Description: Rabbit Polyclonal Antibody Serum to Growth Hormone
  • Application: WB, ELISA
  • Reactivity: Human
  • Conjugate: Unconjugated
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Description

Description: Anti-Human Growth Hormone Serum is developed in rabbit using recombinant Growth Hormone produced in plants.

Immunogen: Highly pure (>97%) recombinant human Growth Hormone expressed in plants.

Applications:

  • ELISA: Suggested starting dilution 1/4,000.  This antibody, in conjunction with compatible secondary reagents (anti-rabbit IgG-AP conjugate), allows the detection of  < 5 ng/well of rhuman Growth Hormone protein (Agrenvec RF0011).
  • Western Blot: Suggested starting dilution 1/5,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
RF0049.-WB-Anti-human-Growth-Hormone-Serum-163x300
Fig 1. Western Blot analysis of rhuman Growth Hormone using  Anti-Human Growth Hormone Serum (RF0049)
Human Growth Hormone  protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:10,000 of Anti-Human Growth Hormone Serum. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM. Lane 2:  contains 0,05 ug of rHuman Growth Hormone  protein.

Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.

Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.