Anti-Human GM-CSF (Serum) – RF0098

SKU:
RF0098
  • Description: Rabbit Polyclonal Antibody Serum to GM-CSF
  • Application: WB, ELISA
  • Reactivity: Human
  • Conjugate: Unconjugated
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Description

Description: Anti-Human GM-CSF Serum is developed in rabbit using recombinant GM-CSF produced in plants.

Immunogen: Highly pure (>97%) recombinant human GM-CSF expressed in plants.

Applications:

  • ELISA: Suggested starting dilution 1/4000. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of  > 0,5 ng/well of rhuman GM-CSF (RF0036).
  • Western Blot: Suggested starting dilution 1/5,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
RF0098.-WB-Anti-Human GM-CSF Serum 
‘Fig 1. Western Blot analysis of rhuman GM-CSF using Anti-Human GM-CSF Serum (RF0098)
‘Human GM-CSF protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1: 4,000 of Anti-Human GM-CSF Serum. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM (kDa); Lane 2: 0.5 ug of rHuman GM-CSF protein.

Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.

Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.