Description: Anti-Human GM-CSF IgG is developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).
Immunogen: Highly pure (>97%) recombinant human GM-CSF expressed in plants.
ELISA: Suggested starting dilution 1/4,000. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of > 0,5 ng/well of rhuman GM-CSF (RF0036).
Western Blot: Suggested starting dilution 1/5,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
‘Fig 1. Western Blot analysis of rhuman GM-CSF using Anti-Human GM-CSF IgG (RF0097)
Human GM-CSF protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1: 1,000 of Anti-Human GM-CSF IgG. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM (kDa); Lane 2: 0.5 ug of rHuman GM-CSF protein.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.Optimal dilution conditions should be determined by the final user. Bulk sizes are available upon request. For R&D purposes only. Purchaser must determine the suitability of the product(s) for their particular use.