Anti-Human Follistatin (Serum) – RF0061

150€

SKU:
RF0061
  • Description: Rabbit Polyclonal Antibody Serum to Follistatin
  • Application: WB, ELISA
  • Reactivity: Human
  • Conjugate: Unconjugated
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Description

Description: Anti-Human Follistatin Serum is developed in rabbit using recombinant rH Follistatin produced in plants.

Immunogen: Highly pure (>97%) recombinant human Follistatin expressed in plants.

Applications:

  • ELISA: Suggested starting dilution 1/500. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of <10 ng/well of rhuman Follistatin (RF0043).
  • Western Blot: Suggested starting dilution 1/500 anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.

Fig 1. Western Blot analysis of rhuman Follistatin using Anti-Human Follistatin Serum (RF0061)
Human Follistatin protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:1,000 of Anti-Human Follistatin Serum.  Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 0.25 ug f non glycosylated rHuman Follistatin protein produced in a prokaryotic expression system. Lane 3: 0.25 ug  of glycosylated isoforms of rH Follistatin produced in an eukaryotic expression system.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.