Anti-Human FLt3 (Serum) – RF0059

150€

SKU:
RF0059
  • Description: Rabbit Polyclonal Antibody Serum to FLt3
  • Application: WB, ELISA
  • Reactivity: Human
  • Conjugate: Unconjugated
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Description

Description: Anti-Human FLt3  Serum is developed in rabbit using recombinant rH FLt3  produced in plants.

Immunogen: Highly pure (>97%) recombinant human FLt3 in expressed in plants.

Applications:

  • ELISA: Suggested starting dilution 1/2,000. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of < of 10 ng/well  of rhuman FLt3  (RF0041).
  • Western Blot: Suggested starting dilution 1/1,000 anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
RF0059.-WB-Anti-Human-FLt3-Serum-267x300
Fig 1. Western Blot analysis of rhuman FLt3 using Anti-Human FLt3 Serum (RF0059)
Human FLt3  protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1: 2,000 of Anti-Human FLt3 Serum.  Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 0.25 ug f non glycosylated rHuman Follistatin protein produced in a prokaryotic expression system. Lane 3: 0.25 ug of  rH Flt3-ligand produced in the endosperm tissue of barley grain.  Lane 4: 0.5 ug of glycosylated isoforms of rH FLt3 produced in an eukaryotic expression system.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.