Anti-Human BAFF (Serum) – RF0035

150€

SKU:
RF0035
  • Description: Rabbit Polyclonal Antibody Serum to BAFF
  • Application: WB, ELISA
  • Reactivity: Human
  • Conjugate: Unconjugated
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Description

Description: Anti-Human BAFF Serum is developed in rabbit using recombinant BAFF produced in plants.

Immunogen: Highly pure (>97%) recombinant human BAFF expressed in plants.

Applications:

  • ELISA: Suggested starting dilution 1/4,000. This antibody, in conjunction with compatible secondary reagents (anti-rabbit IgG-AP conjugate), allows the detection of < of 10 ng/well of rhuman BAFF (RF0030).
  • Western Blot: Suggested starting dilution 1/5,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
RF0035-WB-Anti-Human-BAFF-Serum-263x300
Fig 1. Western Blot analysis of rhuman BAFF using Anti-Human BAFF Serum (RF0035).
Human BAFF protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:10,000 of Anti-Human BAFF Serum. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. Lane 1: MWM; Lane 2: 1 ug of glycosylated isoforms of rH BAFF produced in an eukaryotic expression system. Lane 3: 0.25 ug of non glycosylated rHuman BAFF protein produced in a prokaryotic expression system.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user.
Bulk sizes are available upon request. For R&D purposes only.
Purchaser must determine the suitability of the product(s) for their particular use.