Anti-Human Activin A (Serum) – RF0052

150€

SKU:
RF0052
  • Description: Rabbit Polyclonal Antibody Serum to Activin A
  • Application: WB, ELISA
  • Reactivity: Human
  • Conjugate: Unconjugated
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Description

Description: Anti-Human Activin A Serum is developed in rabbit using recombinant Activin A produced in plants.

Immunogen: Highly pure (>97%) recombinant human Activin A expressed in plants.

Applications:

  • ELISA: Suggested starting dilution 1/1,000. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of 0.2-1 ng/well of rhuman Activin A (RF0010).
  • Western Blot: Suggested starting dilution 1/10,000. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
rf0052Fig 1. Western Blot analysis of rhuman Activin A using Anti-Human Activin A Serum (RF0052)
Human Activin A protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:10,000 of Anti-Human Activin A Serum. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent.  Lane 1: MWM; Lane 2: 0.1 ug of rH Activin A protein.
Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.
Optimal dilution conditions should be determined by the final user. Bulk sizes are available upon request. For R&D purposes only. Purchaser must determine the suitability of the product(s) for their particular use