Anti-Human Activin A (IgG) – RF0016

100€

SKU:
RF0016
  • Description: Rabbit Polyclonal Antibody (IgG) to Activin A
  • Application: WB, ELISA, Neu
  • Reactivity: Human
  • Conjugate: Unconjugated
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Description

Description: Anti-Human Activin A IgG developed in rabbit and purified by affinity chromatography on protein G (> 98% purity).

Immunogen: Highly pure (>97%) recombinant human Activin A expressed in plants.

Applications:

  • ELISA: Suggested starting dilution 1/500. This antibody, in conjunction with compatible secondary reagent (anti-rabbit IgG-AP conjugate), allows the detection of 0.2-1 ng/well of rhuman Activin A (RF0010)
  • Western Blot: Suggested starting dilution 1/500. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) is recommended as secondary reagent.
  • NeutralizationTo yield one-half maximal inhibition (ND50) of the biological activity of Activin A (7.5 ng/ml), a concentration of 60-200 ng/ml of this antibody is required.
rf0016
Fig. Western Blot analysis of rhuman Activin A using Anti-Human Activin A IgG (RF0016).
Human Activin A protein was resolved by SDS-PAGE, transferred to a NC membrane and probed with a dilution 1:1,000 Anti-Human Activin A IgG. Anti-rabbit IgG-AP (alkaline phosphatase conjugate) was used as secondary reagent. MWM;  Lane 1: 0.2 ug of rH Activin A protein.

Where this antibody has not been tested for use in a particular technique this not necessarily excludes its use in such procedures.

Optimal dilution conditions should be determined by the final user.

Bulk sizes are available upon request.
For R&D purposes only. Purchaser must determine the suitability of the product(s) for their particular use.