Molecular weight: Recombinant human full length VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains (amino acids 27-191 P15692-4 VEGFA_HUMA) with His tag at N-terminal.
Description: Vascular endothelial growth factor is a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells in vitro and a strong angiogenic factor in vivo. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. In addition to its action as a mitogen it is a potent vascular permeability
factor (VPF) in vivo and is also a chemo attractant for monocytes cells.
VEGF binds to the FLT1/VEGFR1 and KDR/VEGFR2 present on endothelial cells. Substantial evidence implicates VEGF in the induction of tumor metastasis by stimulating the production of matrix metalloproteinases (MMPs).
Figure 1. SDS-PAGE analysis of recombinant VEGFA. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane 4 MWM: Molecular weight marker (kDa); Lane 1-3 contains 0.3, 0.6 and 1 ug of rhuman VEGF. The recombinant protein migrate with an apparent molecular mass of 19-22 kDa under reducing conditions.
Figure 2.- Analysis of rhuman VEGF with specific antibody by Western Blot; Lane 1: Molecular weight marker (MWM; kDa); Lane 1 contains 0.3 ug of rhuman VEGF.
1.The specific activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC). ED50 1-2 ng/ml
2. Recombinant human VEGF 165a shows effect on kerotinocytes cell proliferation (HaCaT cells).The activity is determined by the dose-dependent stimulation of the cell proliferation 48 hours after treatment with recombinant protein. Cell viability was assessed by MTT . It was observed 30% of cell proliferation with a concentration ˂5ng/ml.
Human recombinant protein expressed in Nicotiana benthamiana.
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