Molecular weight: Recombinant human TRX is a 12.4 kDa protein containing 110 amino acid residues (aa 2 -105 of P10599 THIO_HUMAN) with a His tag N-terminal.
Description: Recombinant human TRX participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. It is a small protein which regulates cellular redox status and scavenges reactive oxygen species (ROS). In additions to its anti-oxidant properties, TRX has a crucial rol in the redox regulation of cellular signalling and activation, it is involved in several redox-dependent processes such as gene expression, signal transduction, cell growth, apoptosis and interacting with variosu kinds of target molecules.
Figure 1. SDS-PAGE analysis of recombinant TRX. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane 1-2 contains 0.1 and 0.2 ug of rhuman TRX, lane 3 Molecular weight marker (MWM; kDa); The recombinant protein migrate as a band between 12.5 kDa under reducing conditions. All bands shown in lane 2 and 3 have been identified by MALDI-TOFF as human TRX.
Figure 2.- Western Blot analysis of recombinant TRX. Lane 1, Molecular weight marker (MWM; kDa), lanes 2-3 contains 0.1 and 0.2 ug of rhuman TRX. The recombinant protein migrate as a band between 12.5 kDa under reducing conditions. All bands shown in lane 2 and 3 have been identified by MALDI-TOFF as human TRX.
1. Specific activity of rHuman TRX is 5-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Human recombinant protein expressed in Nicotiana benthamiana.
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