Active rh TRX – RF0106

  • Product under demand. Please contact us directly at: info@agrenvec.es
  • Description: Active human Thioredoxin (TRX)
    Application: Functional studies, Cell assay, SDS-PAGE, Western Blot, Antibody Production.
  • Alternative Names:   ATL-derived factor, Surface-associated sulphydryl protein
  • UniProtKB: P10599
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Description

Molecular weight: Recombinant human  TRX is a 12.4 kDa protein containing 110 amino acid residues  (aa 2 -105  of P10599 THIO_HUMAN)  with a His tag N-terminal.

Description: Recombinant human TRX  participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. It is a small protein which regulates cellular redox status  and scavenges reactive oxygen species (ROS).  In additions to its anti-oxidant properties, TRX has a crucial rol  in the redox regulation of cellular signalling and activation, it is involved in several redox-dependent processes such as gene expression, signal transduction, cell growth, apoptosis and interacting with variosu kinds of  target molecules.

RF0106-. SDS-PAGE  of recombinant human TRX
Figure 1.  SDS-PAGE analysis of recombinant TRX. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane 1-2 contains 0.1 and 0.2 ug  of rhuman TRX, lane 3 Molecular weight marker (MWM; kDa); The recombinant protein migrate as a band between  12.5 kDa under reducing conditions. All bands shown in lane 2 and 3 have been identified  by MALDI-TOFF as human TRX.
RF0106-Western Blot analysis of recombinant TRX
Figure 2.- Western Blot analysis of recombinant TRX. Lane 1, Molecular weight marker (MWM; kDa), lanes 2-3 contains 0.1 and 0.2 ug  of rhuman TRX. The recombinant protein migrate as a band between  12.5 kDa under reducing conditions. All bands shown in lane 2 and 3 have been identified by MALDI-TOFF as human TRX.

Biological Activity:

1. Specific activity  of rHuman TRX is 5-10 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.

 

Human recombinant protein expressed in Nicotiana benthamiana.

Bulk sizes are available upon request.

For R&D purposes only. Purchaser must determine the suitability of the product(s) for their particular use.

Additional Information

Additional Information
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