Active rh Interleukin 10 – RF0103

  • Product under demand. Please contact us directly at: info@agrenvec.es
  • Description: Active human Interleukin 10
  • Application: BIOASSAY, SDS-PAGE, WB
  • Alternative Names: Cytokine synthesis inhibitory factor, CSIF.
  • UniProtKB: P22301
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Description

Molecular weight: Recombinant human IL-10 is a glycosylated, non-disulfide linked homodimer with an apparent molecular mass of 17 kDa due to glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Description: Interleukin 10 (IL-10) is an anti-inflammatory cytokine initially characterized as a T helper (TH)2 specific cytokine, however, further investigations revealed that IL- 10 production was also associated with T regulatory cell responses. It is now known that almost all cells of both the innate and adaptive arms of the immune system can express IL-10, including dendritic cells (DC), macrophages, mast cells, natural killer cells (NK), eosinophils, neu-trophils, B cells, CD8C T cells, and TH1, TH2, and TH17 CD4C T cells.

IL-10 functions by inhibiting pro-inflammatory cytokines made by macrophages and regulatory T cells including, IFN-γ, TNF-α, IL-2, and IL-3, IL-4, and GM-CSF. IL-10 is also known to suppress antigen presentation on antigen presenting cells. It also stimulates the growth of mast cells, is a cytotoxic T cell differentiation factor, and stimulates B cell differentiation.

RF0103-SDS-PAGE analysis of recombinant Interleukin 10.

Figure 1. SDS-PAGE analysis of recombinant Interleukin-10. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane 1: contains 0.5 ug of recombinant Interleukin 10 (~ 17kDa monomer and ~ 34 kDa homodimers), lane 2: Molecular weight marker (kDa).

Biological Activity:

1. Effect of recombinant Human Interleukin-10 on LPS-induced  kerotinocytes (HaCaT cells).The activity is determined by changes on the gene expression of proinflammatory cytokines IL-6 and TNF-α by qPCR method. mRNA levels of IL-6 and TNF-α cytokines  decrease  between  1-1.5 fold compared with  negative control (not rHuman IL-10 treated cells).

RF0103-The biological activity of Interleukin 10

 

 

 

 

 

 

 

 

 

Human recombinant protein expressed in Nicotiana benthamiana.

Bulk sizes are available upon request.

For R&D purposes only. Purchaser must determine the suitability of the product(s) for their particular use.

 

Additional Information

Additional Information
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