Molecular weight: Recombinant human full length EGF is a 7 kDa globular protein containing 54 amino acid residues (amino acids 971 al 1023 P01133 (EGF_HUMAN) with His tag at N-terminal.
Description: Human Recombinant Epidermal Growth Factor, (rhEGF) is a is a small mitogenic polypeptide which is present in many mammalian species and is distributed throughout a wide number of tissues and body fluids. EGF stimulates the proliferation and differentiation of epithelial cells from skin, cornea, lung and tracheal tissue and the gastrointestinal tract. EGF also promotes growth and migration of keratinocytes and enhances the proliferation of fibroblasts and embryonic cells. It is a member of a growth factor family which is characterized by the presence of 6 conserved cysteine motifs that form three disulfide bonds. The biological effects of EGF are mediated by a specific transmembrane receptor (EGF-R). The binding of EGF to EGFR will induce receptor dimerization, which is required for activating the tyrosine kinase in the receptor cytoplasmic domain. Thus, EGF triggers several signal transduction pathways including JAK/STAT, Ras/ERK and PI3K/AKT pathways.
EGF plays an important role in wound healing and organogenesis .In addition to its proliferative effects, it participle of a variety of other bioactivities, including effects on cytoskeletal organization, cell migration and the synthesis and turnover of extracellular matrix molecules. Therefore, hEGF has wide application prospects in clinical and cosmetic fields.
Figure 1. SDS-PAGE analysis of recombinant EGF. Samples were loaded in 15% SDS-polyacrylamide gel and stained with Coomassie blue. Lane 1: Molecular weight marker (MWM; kDa); Lane 2 contains 0.25 ug of rhuman EGF. The recombinant protein migrate with an apparent molecular mass of 7 kDa under reducing conditions.
Figure 2.- Analysis of rhuman EGF with specific antibody by Western Blot; Lane 1: Molecular weight marker (MWM; kDa); Lane 2 contains 0.25 ug of rhuman EGF.
1.The specific activity is determined by the dose-dependent proliferation of murine BALB/c 3T3 cells (measured by MTT assay). ED50 ˂1 ng/ml
Human recombinant protein expressed in Nicotiana benthamiana.
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